Yiyi (Jessica) Li ’18

Major

Chemistry

Project Title

Identifying Novel Regulators of T-cell Differentiation in MRSA and Influenza Infections

Over the summer, I interned in Dr. Warren Leonard’s molecular immunology lab. My main project involved the study of differences in T-cell differentiation given the presence of a transcription factor that induce multiple sclerosis. For this project, I isolated and differentiated CD4+ T-cells from primary tissue derived from both wild type and knockdown mice. We then observed the differences in the percentages of naïve CD4+ T-cells that had successfully differentiated into two T-cell lineages using fluorescence labeling and flow cytometry. This research contributes not only to our understanding of multiple sclerosis, but also how T-cells function on a more fundamental level. These insights are applicable to other disease conditions as well. During this project, I walked through a portion of the scientific process, particularly the steps of coming up with an investigatable question and trouble-shooting the protocol – skills which will undoubtedly give me tremendous help in my future research projects.



Internship Year

2016

Project Category

Health

Organization(s)

National Institute of Health (NIH), Washington D.C.

Mentor(s)

Warren Leonard, National Institute of Health