Sasha Mykhantso ’27, Molecular Biology

Sasha Mykhantso ’27

Major

Molecular Biology

Project Title

Molecular Biology and Chemistry of the Nitrogen Cycle

Presentation Link

View Sasha's Presentation

Minor(s): Bioengineering

I spent nine weeks analyzing water samples from a recent cruise in the Eastern Tropical South Pacific Ocean. The expedition’s primary objective was to study nitrogen cycling in regions where oxygen levels are minimal, which enables anaerobic processes like denitrification to occur. I investigated the chemistry of ocean water samples and the genomes of microbes. I extracted, purified and quantified DNA and RNA from more than a hundred samples of particulate material captured on filters. Furthermore, I optimized and performed vast numbers of colorimetric and fluorometric assays on the water samples in order to measure the concentration of three major nitrogen nutrients: nitrite, urea and ammonium. The data I produced resulted in nutrient profiles for locations in the ocean with varying conductivity, temperature, depth and oxygen concentrations. Such profiles are essential for learning about the denitrification process performed by the special enzymes produced by the microbes in this region. This invaluable experience working full-time as a researcher has allowed me to solidify my interest in lab work while combining my passions for biochemistry and environmental studies.