Controls on Alternative N2 Fixation: Consequences for Land Sequestration of Anthropogenic CO2 and Bio-hydrogen Production
2015 New Investigator Award
Award Period: 2015-2017
The controls on biological N2 fixation, a key reaction governing the fertility of natural and managed ecosystems, remain poorly understood. Biological N2 fixation is catalyzed by the nitrogenase enzyme that exists in the canonical form containing the metal molybdenum (Mo) and alternative forms containing vanadium (V) or only iron (Fe) in the active site. Using a new experimental method, researchers Anne Morel-Kraepiel and Xinnang Zhang were able to demonstrate for the first time that the alternative V-nitrogenase and the Fe-only nitrogenase make significant contributions to N2 fixation in various ecosystems. This finding calls into question the understanding of the controls on alternative nitrogenases. Their preliminary data indicate that the redox status of the N2-fixing bacteria may control the form of nitrogenase used, in addition to the well-studied conditions of metal availability and/or temperatures. They are currently investigating the interplay of temperature, metal availability and cellular redox status in controlling the type of nitrogenase that is used by N2-fixers. This work will provide a much-needed understanding of the controls on nitrogen fixation with potential for the long-term storage of anthropogenic CO2 on land and the development of strategies for biohydrogen production.
This research project is carried out in part by Carol Chui, an undergraduate student doing a lab internship in the Morel lab over the summer of 2015 and continuing for her senior thesis. Carol is directly supervised by Xinning Zhang. In addition, the team believe that this project, with its potential application to the production of clean alternative energy, will be highly attractive to students interested in acquiring a first experience with laboratory-based research in the fields of energy and the environment.
- Carol Chui, Class of 2016