Kaeli Ficco ’24

Major

Chemical and Biological Engineering

Project Title

Probing Suppression and Evasion of the Plant Immune System by Commensals From the Plant Root Microbiome

Presentation Link

View Kaeli's Presentation

I studied how root commensal bacteria can suppress the plant immune system to enable these bacteria to cohabitate with the plant. Specifically, I investigated a group of bacteria that share a similar protein that cuts an immune-triggering microbe-associated molecular pattern. I was tasked with deleting these genes and using various assays to quantitatively and qualitatively classify immune suppression of my gene deletion strains compared to the wild-type bacteria. Throughout the internship, I utilized and developed multiple lab procedures including cloning, DNA and protein expression techniques, protein separation techniques and techniques to identify plant immune activation. I also learned valuable scientific presentation and writing skills that I look forward to utilizing as I continue my research in the Conway Lab next year.



Internship Year

2023

Project Category

Food Systems and Health

Organization(s)

Conway Lab, Department of Chemical and Biological Engineering, Princeton University - Princeton, New Jersey

Mentor(s)

Jonathan Conway, Assistant Professor of Chemical and Biological Engineering; Samuel Eastman, Postdoctoral Research Associate, Chemical and Biological Engineering